Biological Activities and Stability of a Standardized Pentacyclic Triterpene Enriched Centella asiatica Extract
Natural Product Sciences
;
: 20-24, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-178615
ABSTRACT
Pentacyclic triterpenes, mainly, asiatic acid, madecassic acid, asiaticoside, and madecassoside are the active constituents of Centella asiatica. A pentacyclic triterpene enriched C. asiatica extract (PRE) was prepared and standardized to contain a total pentacyclic triterpenes not less than 65% w/w. This work was focused on determination of antiinflammatory, antioxidant, and tyrosinase inhibitory activities of PRE and its stability. The PRE exhibited a satisfactory nitric oxide inhibitory effect, with an IC50 value of 64.6 µg/mL. In addition, the PRE inhibited tyrosinase enzyme activity with an IC50 value of 104.8 µg/mL. In contrast, the PRE possessed only weak antioxidant activity. The PRE was stable over a period of four months when stored as a dried powder but only in a well-closed container protected from light at 4 °C. An aqueous alcoholic solution of the PRE was stable at pH values of 5.8 and 7.0, but was not stable at a pH of 8.2. Preparations of the PRE in an aqueous solution should be performed in acidic or neutral conditions.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Monofenol Monooxigenasa
/
Concentración 50 Inhibidora
/
Centella
/
Triterpenos Pentacíclicos
/
Alcohólicos
/
Concentración de Iones de Hidrógeno
/
Óxido Nítrico
Límite:
Humanos
Idioma:
Inglés
Revista:
Natural Product Sciences
Año:
2016
Tipo del documento:
Artículo
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