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Protein methylation in cellular proliferation and differentiation: Non-histone nuclear methyl acceptor protein(s) during 3'-methyl-4-dimethylaminoazobenzeneinduced hepatocarcinogenesis
Experimental & Molecular Medicine ; : 35-43, 1997.
Artículo en Inglés | WPRIM | ID: wpr-179546
ABSTRACT
An accelerating effect of methyl-deficient diet (MDD) on hepatocarcinogenesis and methylation pattern of nuclear protein(s) by S-adenosylmethionine protein arginine N-methyltransferase (protein methylase I, PM-I) have been studied with 3'-methyl-4-dimethyl- aminoazobenzene(MeDAB)-treated rats. The MDD+MeDAB-fed group produced typical cancer cells in the liver almost two weeks earlier than the control synthetic diet (CSD)+MeDAB-fed group. Protein methylase I (PM-I) activity in the livers of MDD alone fed rats began to increase at around 2 weeks after MDD-feeding, reaching a peak at 4 weeks and declining thereafter. When nuclei isolated either from normal livers or from cholangiocarcinoma cells were incubated with PM-I preparation from normal liver, 16 and 23-kDa nuclear proteins were the major methylated proteins, regardless of the source of the nuclei. However, when the above mentioned nuclei were incubated with PM-I preparations either from MDD alone fed livers or MDD+ MeDAB-induced cholangiocarcinoma cells, the methylation of 23-kDa protein was not detected. The result suggests that there is a hitherto-unknown PM-I specific to 23 kDa nuclear protein which was lost during methyl deficient diet feeding and hepatocarcinogenesis. The N-terminal 20 amino acids sequence of the 23-kDa protein was found to be (1)Gly-Val-Pro-Leu-(5)X-Arg-Leu-Phe-Asp-(10)His-Ala-Met-Leu-Gln-(15)Ala -His-Arg-Ala-His-(20)Glu, having 94.7% sequence homology with human chorionic somatomammotropin precursor A and B.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Arginina / Lactógeno Placentario / Proteína-Arginina N-Metiltransferasas / Proteína Metiltransferasas / S-Adenosilmetionina / Proteínas Nucleares / Carcinógenos / Alimentos Formulados / Diferenciación Celular / División Celular Límite: Animales Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 1997 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Arginina / Lactógeno Placentario / Proteína-Arginina N-Metiltransferasas / Proteína Metiltransferasas / S-Adenosilmetionina / Proteínas Nucleares / Carcinógenos / Alimentos Formulados / Diferenciación Celular / División Celular Límite: Animales Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 1997 Tipo del documento: Artículo