In vitro inhibition of 10-formyltetrahydrofolate dehydrogenase activity by acetaldehyde
Nutrition Research and Practice
;
: 195-199, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-180634
ABSTRACT
Alcoholism has been associated with folate deficiency in humans and laboratory animals. Previous study showed that ethanol feeding reduces the dehydrogenase and hydrolase activity of 10-formyltetrahydrofolate dehydrogenase (FDH) in rat liver. Hepatic ethanol metabolism generates acetaldehyde and acetate. The mechanisms by which ethanol and its metabolites produce toxicity within the liver cells are unknown. We purified FDH from rat liver and investigated the effect of ethanol, acetaldehyde and acetate on the enzyme in vitro. Hepatic FDH activity was not reduced by ethanol or acetate directly. However, acetaldehyde was observed to reduce the dehydrogenase activity of FDH in a dose- and time-dependent manner with an apparent IC50 of 4 mM, while the hydrolase activity of FDH was not affected by acetaldehyde in vitro. These results suggest that the inhibition of hepatic FDH dehydrogenase activity induced by acetadehyde may play a role in ethanol toxicity.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxidorreductasas
/
Leucovorina
/
Concentración 50 Inhibidora
/
Etanol
/
Alcoholismo
/
Ácido Fólico
/
Oxidorreductasas actuantes sobre Donantes de Grupo CH-NH
/
Hígado
/
Acetaldehído
/
Animales de Laboratorio
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Nutrition Research and Practice
Año:
2008
Tipo del documento:
Artículo
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