Inhibitory Effect of Angiotensin Blockade on Hepatic Fibrosis in Common Bile Duct-ligated Rats / 대한간학회지
The Korean Journal of Hepatology
;
: 61-69, 2007.
Artículo
en Coreano
| WPRIM
| ID: wpr-182808
ABSTRACT
BACKGROUNDS AND AIMS:
Angiotensin receptors are found on hepatic stellate cells, which participate in hepatic fibrosis. Therefore, it is presumed that angiotensin has a role in hepatic fibrosis. The aim of this study was to evaluate the effects of angiotensin blockade on inhibition of hepatic fibrosis in cirrhotic rat model. Material andMETHODS:
Cirrhosis with portal hypertension was produced by common bile duct ligation (BDL) in the adult Sprague-Dawley rats. They were classified into 4 groups (each group n=6) as follows; G1 BDL without drug, G2 BDL+captopril 100 mg/kg/day beginning 2 weeks after BDL, G3 BDL+captopril 100 mg/kg/day, starting just after BDL, G4 BDL+losartan 10 mg/kg/day, starting just after BDL. After 4 weeks following BDL, hepatic fibrosis was histomorphologically analyzed by Batts & Ludwig score. Alpha smooth muscle actin by immunohistochemical stain, hydroxyproline contents of liver tissue by spectrophotometry and expression of collagen, procollagen, and TGF-beta by real-time PCR were measured.RESULTS:
Batts & Ludwig score were 3.8, 3.0, 2.6,and 2.6 in G1, G2, G3, and G4, respectively. The expression of alpha-SMA was significantly lower in G3 and G4 than in G1; 11.9%, 10.9%, 2.6%, and 1.1% in G1, G2, G3, and G4, respectively (p<0.05). The concentration of hydroxyproline (microgram/g liver tissue) was lower in G3 and G4 compared with G1 (p<0.05). Also, the administration of angiotensin blockade just after BDL significantly reduced the expression of collagen, procollagen, and TGF-beta mRNA.CONCLUSIONS:
Angiotensin blockades are effective in the prevention of hepatic fibrosis in BDL rats.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Conductos Biliares
/
Fibrosis
/
Captopril
/
Factor de Crecimiento Transformador beta
/
Actinas
/
Ratas Sprague-Dawley
/
Losartán
/
Bloqueadores del Receptor Tipo 1 de Angiotensina II
/
Hidroxiprolina
/
Ligadura
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Coreano
Revista:
The Korean Journal of Hepatology
Año:
2007
Tipo del documento:
Artículo
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