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Gamma Linolenic Acid Exerts Anti-Inflammatory and Anti-Fibrotic Effects in Diabetic Nephropathy
Yonsei Medical Journal ; : 1165-1175, 2012.
Artículo en Inglés | WPRIM | ID: wpr-183497
ABSTRACT

PURPOSE:

This study was undertaken to investigate the effects of gamma linolenic acid (GLA) on inflammation and extracellular matrix (ECM) synthesis in mesangial and tubular epithelial cells under diabetic conditions. MATERIALS AND

METHODS:

Sprague-Dawley rats were intraperitoneally injected with either a diluent [n=16, control (C)] or streptozotocin [n=16, diabetes (DM)], and eight rats each from the control and diabetic groups were treated with evening primrose oil by gavage for three months. Rat mesangial cells and NRK-52E cells were exposed to medium containing 5.6 mM glucose and 30 mM glucose (HG), with or without GLA (10 or 100 microM). Intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1), and fibronectin (FN) mRNA and protein expression levels were evaluated.

RESULTS:

Twenty-four-hour urinary albumin excretion was significantly increased in DM compared to C rats, and GLA treatment significantly reduced albuminuria in DM rats. ICAM-1, MCP-1, FN mRNA and protein expression levels were significantly higher in DM than in C kidneys, and these increases were significantly abrogated by GLA treatment. In vitro, GLA significantly inhibited increases in MCP-1 mRNA expression and protein levels under high glucose conditions in HG-stimulated mesangial and tubular epithelial cells (p<0.05, respectively). ICAM-1 and FN expression showed a similar pattern to the expression of MCP-1.

CONCLUSION:

GLA attenuates not only inflammation by inhibiting enhanced MCP-1 and ICAM-1 expression, but also ECM accumulation in diabetic nephropathy.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ensayo de Inmunoadsorción Enzimática / Western Blotting / Fibronectinas / Ratas Sprague-Dawley / Ácido alfa-Linolénico / Molécula 1 de Adhesión Intercelular / Quimiocina CCL2 / Nefropatías Diabéticas / Reacción en Cadena en Tiempo Real de la Polimerasa / Antiinflamatorios Límite: Animales Idioma: Inglés Revista: Yonsei Medical Journal Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ensayo de Inmunoadsorción Enzimática / Western Blotting / Fibronectinas / Ratas Sprague-Dawley / Ácido alfa-Linolénico / Molécula 1 de Adhesión Intercelular / Quimiocina CCL2 / Nefropatías Diabéticas / Reacción en Cadena en Tiempo Real de la Polimerasa / Antiinflamatorios Límite: Animales Idioma: Inglés Revista: Yonsei Medical Journal Año: 2012 Tipo del documento: Artículo