Induction of IL-8 and reactive oxygen species in periodontal ligament cells by Aggregatibacter actinomycetemcomitans / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 331-337, 2009.
Artículo
en Coreano
| WPRIM
| ID: wpr-183844
ABSTRACT
PURPOSE:
Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O2. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells.METHODS:
The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS.RESULTS:
A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-cetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans.CONCLUSION:
These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ligamento Periodontal
/
Ensayo de Inmunoadsorción Enzimática
/
Citocinas
/
Interleucina-8
/
Interleucinas
/
Especies Reactivas de Oxígeno
/
Fluoresceínas
Idioma:
Coreano
Revista:
The Journal of the Korean Academy of Periodontology
Año:
2009
Tipo del documento:
Artículo
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