Evaluation of Her-2/neu in Breast Cancer: Comparison of Immunohistochemistry and Polymerase Chain Reaction / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 96-101, 2003.
Artículo
en Coreano
| WPRIM
| ID: wpr-18461
ABSTRACT
PURPOSE:
In breast cancer, Her-2/neu amplification/overexpression predicts a poor clinical outcome, and enhanced survival benefits have been reported with Her-2/neu targeted therapy. Currently, there are several methods for assessing the amplification/overexpression of Her-2/neu, each having advantages and disadvantages. The aim of this work was to establish a reproducible, sensitive and specific method of testing for Her-2/neu, which could be used in diagnostic pathology laboratories. MATERIALS ANDMETHODS:
We compared the immunohistochemistry (IHC) detection of Her-2/neu overexpression, with differential polymerase chain reaction (PCR) to assess the gene amplification of the Her-2/ neu, in 163 cases of invasive ductal carcinoma of the breast using paraffin-embedded tissue. In addition, assessment of the appropriate cut off points was established.RESULTS:
The overexpression of the Her-2/neu was detected in 39 (23.9%) cases, and its amplification in 37 (22.7%) cases. The methods were positive in 21.5% of cases and negative in 74.8%. There was a 96.3% concordance between the two methods. The sensitivity and specificity of IHC, compared with PCR, were 94.6 and 96.8%, respectively.CONCLUSION:
We conclude that the automation of PCR-based Her-2/neu testing approaches is expected to play an increasing role in the future of Her-2/neu testing. Also, we have demonstrated that IHC is a sensitive and specific method for assessing Her-2/neu stati in breast cancer, compared to PCR. The current study indicates that moderate, or strong, complete membrane staining in> or =10% of tumor cells provides an appropriate cut off point compared with PCR.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Automatización
/
Mama
/
Neoplasias de la Mama
/
Inmunohistoquímica
/
Amplificación de Genes
/
Reacción en Cadena de la Polimerasa
/
Sensibilidad y Especificidad
/
Carcinoma Ductal
/
Membranas
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Idioma:
Coreano
Revista:
Cancer Research and Treatment
Año:
2003
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS