In vitro MRI and Characterization of Rat Mesenchymal Stem Cells Transduced with Ferritin as MR Reporter Gene
Journal of the Korean Society of Magnetic Resonance in Medicine
; : 47-54, 2012.
Article
en En
| WPRIM
| ID: wpr-185403
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: This study was performed to evaluate the characteristics of rat mesenchymal stem cells (RMSCs) transduced with human ferritin gene and investigate in vitro MRI detectability of ferritin-transduced RMSCs. MATERIALS AND METHODS: The RMSCs expressing both myc-tagged human ferritin heavy chain subunit (myc-FTH) and green fluorescence protein (GFP) were transduced with lentiviurs. Transduced cells were sorted by GFP expression using a fluorescence-activated cell sorter. Myc-FTH and GFP expression in transduced cells were detected by immunofluorescence staining. The cell proliferative ability and viability were assessed by MTT assay. The RMSC surface markers (CD29+/CD45-) were analyzed by flow cytometry. The intracellular iron amount was measured spectrophotometically and the presence of ferritin-iron accumulation was detected by Prussian blue staining. In vitro magnetic resonance imaging (MRI) study of cell phantoms was done on 9.4 T MR scanner to evaluate the feasibility of imaging the ferritin-transduced RMSCs. RESULTS: The myc-FTH and GFP genes were stably transduced into RMSCs. No significant differences were observed in terms of biologic properties in transduced RMSCs compared with non-transduced RMSCs. Ferritin-transduced RMSCs exhibited increased iron accumulation ability and showed significantly lower T2 relaxation time than non-transduced RMSCs. CONCLUSION: Ferritin gene as MR reporter gene could be used for non-invasive tracking and visualization of therapeutic mesenchymal stem cells by MRI.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Apoferritinas
/
Relajación
/
Atletismo
/
Imagen por Resonancia Magnética
/
Técnica del Anticuerpo Fluorescente
/
Genes Reporteros
/
Ferritinas
/
Ferrocianuros
/
Células Madre Mesenquimatosas
/
Citometría de Flujo
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Journal of the Korean Society of Magnetic Resonance in Medicine
Año:
2012
Tipo del documento:
Article