Co-activation of Gi and Gq proteins exerts synergistic effect on human platelet aggregation through activation of phospholipase C and Ca2+ signalling pathways
Experimental & Molecular Medicine
; : 42-46, 1999.
Article
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| ID: wpr-186198
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WPRO
ABSTRACT
Our previous studies have shown that subthreshold concentrations of two platelet agonists exert synergistic effects on platelet aggregation. Here we studied the mechanism of synergistic interaction of 5-hydroxytryptamine (5-HT) and epinephrine mediated platelet aggregation. We show that 5-HT had no or little effect on aggregation but it did potentiate the aggregation response of epinephrine. The synergistic interaction of 5-HT (1-5 microM) and epinephrine (0.5-2 microM) was inhibited by alpha2-adrenoceptor blocker (yohimbine; IC50= 0.4 microM), calcium channel blockers (verapamil and diltiazem with IC50 of 10 and 48 mM, respectively), PLC inhibitor (U73122; IC50=6 microM) and nitric oxide (NO) donor, SNAP (IC50=1.6 microM)). The data suggest that synergistic effects of platelet agonists are receptor-mediated and occur through multiple signalling pathways including the activation PLC/Ca2+ signalling cascades.
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Texto completo:
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Índice:
WPRIM
Asunto principal:
Fosfolipasas de Tipo C
/
Bloqueadores de los Canales de Calcio
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Transducción de Señal
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Epinefrina
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Serotonina
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Agregación Plaquetaria
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Western Blotting
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Proteínas de Unión al GTP
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Subunidades alfa de la Proteína de Unión al GTP Gi-Go
/
Señalización del Calcio
Límite:
Humans
Idioma:
En
Revista:
Experimental & Molecular Medicine
Año:
1999
Tipo del documento:
Article