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N-acetyl-D-glucosamine kinase interacts with dynein light-chain roadblock type 1 at Golgi outposts in neuronal dendritic branch points
Experimental & Molecular Medicine ; : e177-2015.
Artículo en Inglés | WPRIM | ID: wpr-186434
ABSTRACT
N-acetylglucosamine kinase (GlcNAc kinase or NAGK) is a ubiquitously expressed enzyme in mammalian cells. Recent studies have shown that NAGK has an essential structural, non-enzymatic role in the upregulation of dendritogenesis. In this study, we conducted yeast two-hybrid screening to search for NAGK-binding proteins and found a specific interaction between NAGK and dynein light-chain roadblock type 1 (DYNLRB1). Immunocytochemistry (ICC) on hippocampal neurons using antibodies against NAGK and DYNLRB1 or dynein heavy chain showed some colocalization, which was increased by treating the live cells with a crosslinker. A proximity ligation assay (PLA) of NAGK-dynein followed by tubulin ICC showed the localization of PLA signals on microtubule fibers at dendritic branch points. NAGK-dynein PLA combined with Golgi ICC showed the colocalization of PLA signals with somal Golgi facing the apical dendrite and with Golgi outposts in dendritic branch points and distensions. NAGK-Golgi PLA followed by tubulin or DYNLRB1 ICC showed that PLA signals colocalize with DYNLRB1 at dendritic branch points and at somal Golgi, indicating a tripartite interaction between NAGK, dynein and Golgi. Finally, the ectopic introduction of a small peptide derived from the C-terminal amino acids 74-96 of DYNLRB1 resulted in the stunting of hippocampal neuron dendrites in culture. Our data indicate that the NAGK-dynein-Golgi tripartite interaction at dendritic branch points functions to regulate dendritic growth and/or branching.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Tubulina (Proteína) / Datos de Secuencia Molecular / Células Cultivadas / Secuencia de Aminoácidos / Ratas Sprague-Dawley / Fosfotransferasas (Aceptor de Grupo Alcohol) / Dendritas / Dineínas Citoplasmáticas / Células HEK293 / Mapas de Interacción de Proteínas Límite: Animales / Humanos Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 2015 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Tubulina (Proteína) / Datos de Secuencia Molecular / Células Cultivadas / Secuencia de Aminoácidos / Ratas Sprague-Dawley / Fosfotransferasas (Aceptor de Grupo Alcohol) / Dendritas / Dineínas Citoplasmáticas / Células HEK293 / Mapas de Interacción de Proteínas Límite: Animales / Humanos Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 2015 Tipo del documento: Artículo