Detection of an AB(weak) Blood Group by Genotyping / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 71-76, 2006.
Artículo
en Coreano
| WPRIM
| ID: wpr-187476
ABSTRACT
Since precise ABO blood group typing is the most important part of a safe blood transfusion, the cause of an ABO discrepancy should be determined before issuing blood. The authors confirmed the ABO blood group of a 25-year-healthy male using genotyping method in those who showed an ABO blood group discrepancy between the cell and serum types. ABO typing, saliva test, adsorption elution test and serum transferase assay were performed and the presence of a weak B substance and B transferase was suspected without any evidence of the B antigen on red blood cells. Polymerase-chain-reaction restriction-fragment-length-polymorphism (PCR-RFLP) analysis was performed using the restriction enzymes including BstE II, Pvu II, BssH II, Alu I, and Mva I. The genotyping result showed a normal A allele and B allele. DNA analysis of exons 6 and 7 revealed normal A and B sequences. Therefore, we confirmed A(1)B(weak) blood type.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Saliva
/
Transferasas
/
Transfusión Sanguínea
/
ADN
/
Exones
/
Adsorción
/
Alelos
/
Eritrocitos
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Korean Journal of Blood Transfusion
Año:
2006
Tipo del documento:
Artículo
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