The Role of Focal Adhesion Kinase in the TGF-beta-Induced Myofibroblast Transdifferentiation of Human Tenon's Fibroblasts
Korean Journal of Ophthalmology
;
: 45-48, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-187594
ABSTRACT
PURPOSE:
To investigate the role of focal adhesion kinase (FAK) in transforming growth factor (TGF)-beta-induced myofibroblast transdifferentiation of human Tenon's fibroblasts.METHODS:
Primary cultured human Tenon's fibroblasts were exposed to TGF-beta1 for up to 48 hours. The mRNA levels of FAK, alpha smooth muscle actin (alphaSMA), and beta-actin were determined by quantitative real time reverse transcription polymerase chain reaction. The protein levels of collagen type I, FAK, phospho-FAK, alphaSMA, and beta-actin were determined by Western immunoblots. After the small interfering RNA targeting FAK (siRNA(FAK)) molecules were delivered into the cells, the expressions of alphaSMA proteins were determined by Western immunoblots.RESULTS:
In human Tenon's fibroblasts, TGF-beta1 significantly increased the mRNA and protein expressions of alphaSMA. However, when the action of FAK was inhibited using siRNAFAK, the TGF-beta1-induced expression of alphaSMA was attenuated.CONCLUSIONS:
Our data suggest that FAK may be associated with the TGF-beta1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts, which is the essential step of subconjunctival fibrosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Células Cultivadas
/
Western Blotting
/
Análisis de Varianza
/
Factor de Crecimiento Transformador beta
/
Actinas
/
Colágeno
/
ARN Interferente Pequeño
/
Proteína-Tirosina Quinasas de Adhesión Focal
/
Transdiferenciación Celular
Límite:
Humanos
Idioma:
Inglés
Revista:
Korean Journal of Ophthalmology
Año:
2012
Tipo del documento:
Artículo
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