Alterations in the Kinetics of CD4+ T Cell Responses with Aging / 대한류마티스학회지
The Journal of the Korean Rheumatism Association
;
: 271-280, 2009.
Artículo
en Coreano
| WPRIM
| ID: wpr-187839
ABSTRACT
OBJECTIVE:
Alterations in the immune system occur with aging, and these contribute to an increased risk of infection and malignancy. The age-associated changes in T cell immunity range from single cell function to the maintenance of cell populations. We investigated the kinetics of CD4+ T cell activation and proliferation in young and elderly subjects after stimulating their peripheral blood mononuclear cells with anti-CD3 and anti-CD28 antibodies (Abs).METHODS:
The expressions of the activation markers CD69, CD40L and CD25 on the CD4+ T cells from young (n=14) and elderly (n=19) were analyzed at 6, 24 and 48 hours (hrs) of T cell receptor (TCR) stimulation by using flow cytometry. In the same individuals, the CD4+ T cell proliferation was determined at 48 and 96 hrs of TCR stimulation by using the CFSE dilution method.RESULTS:
The elderly had decreased CD69 and CD40L expressions on the CD4+ T cells at 6 hrs of stimulation, as compared to that of the young patients. The elderly also had a decreased CD25 expression on the CD4+ T cells at 24 hrs of stimulation. However, the two groups had similar levels of the CD25, CD69 and CD40L expressions at 48 hrs of stimulation. The elderly had decreased CD4+ T cell proliferation at 96 hrs of stimulation, as compared to that of the young, although both groups had similar levels of CD4+ T cell proliferation at 48 hrs of stimulation.CONCLUSION:
Our findings suggest that the elderly have altered kinetics of CD4+ T cell activation and proliferation in response to anti-CD3 and -CD28 Ab stimulation, and that such an altered response is governed by the duration of stimulation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Succinimidas
/
Envejecimiento
/
Cinética
/
Receptores de Antígenos de Linfocitos T
/
Linfocitos T
/
Ligando de CD40
/
Proliferación Celular
/
Citometría de Flujo
/
Fluoresceínas
/
Sistema Inmunológico
Límite:
Anciano
/
Humanos
Idioma:
Coreano
Revista:
The Journal of the Korean Rheumatism Association
Año:
2009
Tipo del documento:
Artículo
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