Mesenchymal Stem Cells Improve Wound Healing In Vivo via Early Activation of Matrix Metalloproteinase-9 and Vascular Endothelial Growth Factor
Journal of Korean Medical Science
; : 726-733, 2011.
Article
en En
| WPRIM
| ID: wpr-188471
Biblioteca responsable:
WPRO
ABSTRACT
We investigated the effects of mesenchymal stem cells (MSCs) on wound healing using a three-dimensional (3D) collagen gel scaffold. Three circular full-thickness skin defects were created on the back of Sprague-Dawley rats. One site was covered with a 3D collagen gel containing 2 x 10(6) MSCs (MSCs+/3D collagen+). Another site was replaced with a 3D collagen gel without MSCs and the third site was left empty. The wound size was significantly reduced in the MSCs+/3D collagen+ sites. MSCs+/3D collagen+ sites exhibited the most neovascularization. FISH showed that Y-chromosome possessing cells were found within the dermis of MSCs+/3D collagen+ sites. Gelatin zymography revealed that the most intense expression of MMP-9 was detected early in the MSCs+/3D collagen+ sites. Our results indicate that MSCs upregulate the early expression of MMP-9 which induces the early mobilization of VEGF. Thus, MSCs appear to accelerate significantly wound healing via early activation of MMP-9 and VEGF.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Piel
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Cicatrización de Heridas
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Inmunohistoquímica
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Colágeno
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Ratas Sprague-Dawley
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Neovascularización Fisiológica
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Metaloproteinasa 9 de la Matriz
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Factor A de Crecimiento Endotelial Vascular
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Trasplante de Células Madre Mesenquimatosas
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Células Madre Mesenquimatosas
Límite:
Animals
Idioma:
En
Revista:
Journal of Korean Medical Science
Año:
2011
Tipo del documento:
Article