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Mesenchymal Stem Cells Improve Wound Healing In Vivo via Early Activation of Matrix Metalloproteinase-9 and Vascular Endothelial Growth Factor
Article en En | WPRIM | ID: wpr-188471
Biblioteca responsable: WPRO
ABSTRACT
We investigated the effects of mesenchymal stem cells (MSCs) on wound healing using a three-dimensional (3D) collagen gel scaffold. Three circular full-thickness skin defects were created on the back of Sprague-Dawley rats. One site was covered with a 3D collagen gel containing 2 x 10(6) MSCs (MSCs+/3D collagen+). Another site was replaced with a 3D collagen gel without MSCs and the third site was left empty. The wound size was significantly reduced in the MSCs+/3D collagen+ sites. MSCs+/3D collagen+ sites exhibited the most neovascularization. FISH showed that Y-chromosome possessing cells were found within the dermis of MSCs+/3D collagen+ sites. Gelatin zymography revealed that the most intense expression of MMP-9 was detected early in the MSCs+/3D collagen+ sites. Our results indicate that MSCs upregulate the early expression of MMP-9 which induces the early mobilization of VEGF. Thus, MSCs appear to accelerate significantly wound healing via early activation of MMP-9 and VEGF.
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Texto completo: 1 Índice: WPRIM Asunto principal: Piel / Cicatrización de Heridas / Inmunohistoquímica / Colágeno / Ratas Sprague-Dawley / Neovascularización Fisiológica / Metaloproteinasa 9 de la Matriz / Factor A de Crecimiento Endotelial Vascular / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Journal of Korean Medical Science Año: 2011 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Piel / Cicatrización de Heridas / Inmunohistoquímica / Colágeno / Ratas Sprague-Dawley / Neovascularización Fisiológica / Metaloproteinasa 9 de la Matriz / Factor A de Crecimiento Endotelial Vascular / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Journal of Korean Medical Science Año: 2011 Tipo del documento: Article