A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
The Korean Journal of Parasitology
;
: 503-510, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-189491
ABSTRACT
Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Factores de Tiempo
/
Toxoplasma
/
Uganda
/
Proteínas Recombinantes de Fusión
/
Pruebas Serológicas
/
Anticuerpos Antiprotozoarios
/
Datos de Secuencia Molecular
/
Proteínas Protozoarias
/
Toxoplasmosis
/
Reproducibilidad de los Resultados
Tipo de estudio:
Estudio diagnóstico
Límite:
Adolescente
/
Adulto
/
Niño
/
Child, preschool
/
Femenino
/
Humanos
/
Lactante
/
Masculino
País/Región como asunto:
Africa
/
Asia
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2013
Tipo del documento:
Artículo
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