Production of the Recombinant Single Chain Anti-B Cell Lymphoma Antibody and Evaluation of Immunoreactivity / 핵의학분자영상
Nuclear Medicine and Molecular Imaging
;
: 211-217, 2006.
Artículo
en Coreano
| WPRIM
| ID: wpr-191181
ABSTRACT
PURPOSE:
Recombinant ScFv lym-1 was produced, using pET vector system for large scale production.METHODS:
ScFv lym-1 gene inserted pET-22b (+) vector, was expressed in E.coli BL-21 strain. ScFv lym-1 antibody extracted from periplasm, was purified with His-Taq column. To evaluated immunoreactivity with Raji cell, ScFv lym-1 was labeled with I-125 and I-125 ScFv lym-1 was purified with desalting column. Raji cell was injected into the C57BR/cdJ SCID mice. Gamma camera imaging were taken time point at 1, 8, 24, and 48 hr with 8 mm pinhole collimator.RESULTS:
An active scFv lym-1 could be produced in E.coli with soluble from using pET vector system. Immunoreactivity and affinity constant of IgG lym-1 were 54% and 1.83 x 10(9) M(-1), respectively, and those of scFv lym-1 were 53.7% and 1.46 x 10(9) M(-1), respectively. Biodistribution of I-125 scFv lym-1 antibody showed faster clearance in blood, spleen, kidney and than I-125 IgG lym-1 antibody. Gamma camera image of I-125 scFv lym-1 antibody showed faster clearance and tumor targeting liver than I-125 IgG lym-1 antibody.CONCLUSIONS:
In vitro properties of scFv lym-1 were similar to those of IgG lym-1. ScFv lym-1 showed faster blood clearance than IgG lym-1. These results suggest that scFv lym-1 antibody can be useful for tumor imaging agent.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Bazo
/
Inmunoglobulina G
/
Cintigrafía
/
Cámaras gamma
/
Ratones SCID
/
Periplasma
/
Riñón
/
Hígado
/
Linfoma
Límite:
Animales
Idioma:
Coreano
Revista:
Nuclear Medicine and Molecular Imaging
Año:
2006
Tipo del documento:
Artículo
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