Evaluation of alpha-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia
The Korean Journal of Parasitology
;
: 287-291, 2009.
Artículo
en Inglés
| WPRIM
| ID: wpr-191532
ABSTRACT
The alpha/beta-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant alpha-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of alpha-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the alpha-tubulin gene from G. lamblia. PCR-RFLP analysis of this alpha-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that alpha-tubulin can be used as a molecular probe to detect G. lamblia.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tubulina (Proteína)
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Sondas Moleculares
/
Proteínas Protozoarias
/
Reacción en Cadena de la Polimerasa
/
Alineación de Secuencia
/
Giardiasis
/
Giardia lamblia
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2009
Tipo del documento:
Artículo
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