Production and characterization of monoclonal antibodies to oxidized LDL
Experimental & Molecular Medicine
;
: 41-45, 1998.
Artículo
en Inglés
| WPRIM
| ID: wpr-192958
ABSTRACT
Oxidized low density lipoprotein (LDL) seems to take a part in atherogenesis through direct interactions with macrophages, endothelial cells, and smooth muscle cells, and is thought to participate in renal glomerular injury. For the purpose of illustrating the role of oxidized LDL in the human diseases, monoclonal antibodies were developed and characterized, recognizing oxidized LDL-specific epitopes that do not exist on native LDL. LDL was oxidized by the incubation with CuSO4, and used as immunogen. Splenocytes from the immunized mouse and mouse myeloma cells were fused to produce hybridomas, which were screened for the secretion of oxidized LDL-specific antibodies. Immunoblot analysis and binding affinity assay showed that these monoclonal antibodies recognize malondialdehyde-conjugated peptide epitopes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fragmentos de Péptidos
/
Ensayo de Inmunoadsorción Enzimática
/
Sustancias Reactivas al Ácido Tiobarbitúrico
/
Lipoproteínas LDL
/
Malondialdehído
/
Anticuerpos Monoclonales
/
Afinidad de Anticuerpos
/
Especificidad de Anticuerpos
/
Epítopos
Límite:
Humanos
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
1998
Tipo del documento:
Artículo
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