A mechanism of differential expression of GLUT2 in hepatocyte and pancreatic beta-cell line
Experimental & Molecular Medicine
;
: 15-20, 1998.
Artículo
en Inglés
| WPRIM
| ID: wpr-192962
ABSTRACT
DNase I footprinting assay using liver nuclear extracts revealed six protected regions between nucleotide -600 and +110 and hence named Box I-VI. Upstream promoter element (UPE), a DNA element playing crucial role in transcriptional control of the tissue specific expression of pancreatic beta-cell, has been detected within the proximal region of rat GLUT2 promoter. This region is included in Box VI. The protein-DNA interaction in this region (Box VI) was confirmed by mobility shift assay using liver nuclear extracts. Deletion of the region between -585 bp and -146 bp resulted in dramatic changes in promoter activity when they were expressed in liver and beta-cell derived cell line. When -585/-146 construct was expressed in liver, the activity was decreased to 46%, whereas the activity in beta-cell line, HIT-T15 cell, was increased by 84% when compared to -146/+190 construct. These opposing phenomena can be explained by the fact that beta-cell specifically expresses the UPE binding protein. Assuming that there may be Box VI-binding protein playing negative roles both in hepatocyte and beta-cell, and that the protein acts as a negative regulator of GLUT2 gene, the UPE binding protein in the beta-cell may overcome the inhibition by binding to the protein.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Unión Proteica
/
Sitios de Unión
/
Proteínas de Transporte de Monosacáridos
/
Estudio Comparativo
/
Línea Celular
/
Regulación de la Expresión Génica
/
Islotes Pancreáticos
/
Regiones Promotoras Genéticas
/
Factor de Transcripción AP-1
/
Huella de ADN
Límite:
Animales
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
1998
Tipo del documento:
Artículo
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