Cytotoxicity of resin-based root canal sealer, adseal / 대한치과보존학회지
Journal of Korean Academy of Conservative Dentistry
;
: 498-503, 2004.
Artículo
en Coreano
| WPRIM
| ID: wpr-194028
ABSTRACT
The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex). An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at 37degrees C in humidified 5% CO2-containing air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 mm and height 5 mm placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1, 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Atmósfera
/
Hidróxido de Calcio
/
Tamizaje Masivo
/
Técnicas de Cultivo de Célula
/
Agar
/
Cavidad Pulpar
/
Difusión
/
Fibroblastos
/
Vidrio
Tipo de estudio:
Estudio de tamizaje
Idioma:
Coreano
Revista:
Journal of Korean Academy of Conservative Dentistry
Año:
2004
Tipo del documento:
Artículo
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