Chlorogenic Acid Decreases Retinal Vascular Hyperpermeability in Diabetic Rat Model
Journal of Korean Medical Science
;
: 608-613, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-194140
ABSTRACT
To evaluate the effect of chlorogenic acid (CGA), a polyphenol abundant in coffee, on retinal vascular leakage in the rat model of diabetic retinopathy, Sprague-Dawley rats were divided into four groups controls, streptozotocin-induced diabetic rats, and diabetic rats treated with 10 and 20 mg/kg chlorogenic acid intraperitoneally daily for 14 days, respectively. Blood-retinal barrier (BRB) breakdown was evaluated using FITC-dextran. Vascular endothelial growth factor (VEGF) distribution and expression level was evaluated with immunohistochemistry and Western blot analysis. Expression of tight junction proteins, occludin and claudin-5, and zonula occludens protein, ZO-1 was also evaluated with immunohistochemistry and Western blot analysis. BRB breakdown and increased vascular leakage was found in diabetic rats, with increased VEGF expression and down-regulation of occludin, claudin-5, and ZO-1. CGA treatment effectively preserved the expression of occludin, and decreased VEGF levels, leading to less BRB breakdown and less vascular leakage. CGA may have a preventive role in BRB breakdown in diabetic retinopathy by preserving tight junction protein levels and low VEGF levels.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Retina
/
Barrera Hematorretinal
/
Regulación hacia Abajo
/
Ácido Clorogénico
/
Dextranos
/
Fluoresceína-5-Isotiocianato
/
Ratas Sprague-Dawley
/
Factor A de Crecimiento Endotelial Vascular
/
Diabetes Mellitus Experimental
/
Retinopatía Diabética
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Korean Medical Science
Año:
2013
Tipo del documento:
Artículo
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