Assessment of skin toxicity using living skin equivalents / 대한피부과학회지
Korean Journal of Dermatology
;
: 308-313, 2003.
Artículo
en Coreano
| WPRIM
| ID: wpr-194589
ABSTRACT
BACKGROUND:
There are various alternative in vitro irritancy tests for substituting animal experiment. However, there is no standardized method or guideline for assessing in vitro cytotoxicity.OBJECTIVES:
In order to predict toxicity on human skin, an experimental model was evaluated for the cutaneous cytotoxicity using living skin equivalents(LSE).METHODS:
After applying test substances (SLS, various plant extracts) on LSE, morphological examination and MTT reduction assay was done.RESULTS:
The results showed that cytotoxicity of SLS using LSE was well correlated with the concentration of SLS. Furthermore, characteristic histologic findings were observed according to the increasing concentrations of test materials.CONCLUSION:
These results showed that LSE can be used as an alternative model to replace animal model for cytotoxicity testing.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas
/
Piel
/
Modelos Animales
/
Experimentación Animal
/
Modelos Teóricos
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Dermatology
Año:
2003
Tipo del documento:
Artículo
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