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The Effect of Vasoactive Intestinal Peptide on Cord Blood CD34 (+) Cells / 대한소아혈액종양학회지
Korean Journal of Pediatric Hematology-Oncology ; : 262-268, 2003.
Artículo en Coreano | WPRIM | ID: wpr-194907
ABSTRACT

PURPOSE:

We investigated the expression of vasoactive intestinal peptide (VIP), VIP receptor 1 (VPAC1), VIP receptor 2 (VPAC2) genes in the human umbilical cord blood CD34 cells, and the ability of VIP to stimulate human primitive as well as monopotent hematopoietic progenitors.

METHODS:

We isolated RNA from umbilical cord blood CD34 cells, and then performed RT-PCR, and sequencing. The umbilical cord blood CD34 cells were cultured with the various concentrations of VIP for burst-forming unit of erythrocyte (BFU-E), colony-forming unit of granulocyte/monocyte (CFU-GM), colony-forming unit of graulocyte/erythrocyte/monocyte/megakaryocyte (CFU-GEMM), and colony-forming unit of megakaryocyte (CFU-Mk).

RESULTS:

The RNA coding for VPAC1 was detected in human umbilical cord blood CD34 cells. VIP significantly stimulated the growth of CFU-GEMM and CFU-Mk.

CONCLUSION:

The present results suggest that VIP is an important neuropeptide in the early proliferation of human primitive as well as megakaryocyte progenitors.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células Madre / Neuropéptidos / ARN / Megacariocitos / Péptido Intestinal Vasoactivo / Receptores de Péptido Intestinal Vasoactivo / Células Progenitoras Mieloides / Eritrocitos / Células Progenitoras de Megacariocitos / Sangre Fetal Límite: Humanos Idioma: Coreano Revista: Korean Journal of Pediatric Hematology-Oncology Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células Madre / Neuropéptidos / ARN / Megacariocitos / Péptido Intestinal Vasoactivo / Receptores de Péptido Intestinal Vasoactivo / Células Progenitoras Mieloides / Eritrocitos / Células Progenitoras de Megacariocitos / Sangre Fetal Límite: Humanos Idioma: Coreano Revista: Korean Journal of Pediatric Hematology-Oncology Año: 2003 Tipo del documento: Artículo