Effects of Stress on Mouse beta-Defensin-3 Expression in the Upper Digestive Mucosa
Yonsei Medical Journal
;
: 387-394, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-19550
ABSTRACT
PURPOSE:
Gastrointestinal integrity and immune surveillance are affected by stress. Stress also adversely affects mucosal barrier function. beta-defensins constitute an integral component of the innate immune system as antimicrobial peptides, serving as the first line of defense against microbial pathogens at the epithelial surfaces of the upper digestive mucosa. The primary objective of this study was to determine the effects of stress on the expression profile of mouse beta-defensin-3 in the upper digestive mucosa of mice with diabetes. MATERIALS ANDMETHODS:
We established a mouse model of restraint stress by using NSY/Hos mice with type 2 diabetes mellitus. We used real-time polymerase chain reaction, in situ hybridization, and immunohistochemistry to investigate the effects of stress and glucocorticoid administration on mouse beta-defensin-3 expression in the upper digestive mucosa of the gingiva, esophagus, and stomach.RESULTS:
Mouse beta-defensin-3 mRNA expression was higher in the esophagus than in the gingiva or stomach (p<0.05). In the esophagus, mouse beta-defensin-3 mRNA expression was lower in stressed mice than in non-stressed mice (p<0.05). Furthermore, immunoreactivity to mouse beta-defensin-3 protein was lower in the esophagus of stressed mice than non-stressed mice, consistent with the results of mRNA expression analysis. Systemic glucocorticoid administration also downregulated esophageal mouse beta-defensin-3 mRNA expression.CONCLUSION:
Our novel findings show that stress decreases mouse beta-defensin-3 expression in the esophagus of mice with diabetes, possibly due to increased endogenous glucocorticoid production. It appears to be highly likely that stress management may normalize mucosal antimicrobial defenses in patients with diabetes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Péptidos
/
Estómago
/
ARN Mensajero
/
Inmunohistoquímica
/
Hibridación in Situ
/
Beta-Defensinas
/
Diabetes Mellitus
/
Diabetes Mellitus Tipo 2
/
Esófago
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2014
Tipo del documento:
Artículo
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