Involvement of Oxidative Stress in Cisplatin-Induced Apoptosis in HeLa Cells / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 2410-2416, 2003.
Artículo
en Coreano
| WPRIM
| ID: wpr-196014
ABSTRACT
OBJECTIVE:
To determine whether oxidants are formed as part of the cisplatin-induced apoptotic process, intracellular markers of oxidative stress were examined.METHODS:
Apoptotic death of HeLa cells by cisplatin was confirmed by flow cytometry.RESULTS:
The pre-treatment with glutathione (GSH) significantly attenuated cisplatin-induced apoptosis through the reduction of reactive oxygen species (ROS) accumulation and diminished caspases-3 and 9 protease activity. Furthermore, z-VAD-fmk, an inhibitor of pan-caspase, effectively inhibited the activation of caspases and prevented apoptosis by cisplatin, although cisplatin-induced ROS generation was not attenuated.CONCLUSION:
These data indicate that ROS may play a role as an upstream mediator of caspases. Taken together, our results suggest that oxidative stress mediates cisplatin-induced apoptosis in HeLa cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células HeLa
/
Cisplatino
/
Oxidantes
/
Especies Reactivas de Oxígeno
/
Apoptosis
/
Estrés Oxidativo
/
Caspasas
/
Citometría de Flujo
/
Glutatión
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
2003
Tipo del documento:
Artículo
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