Glucose-Insulin-Potassium Solution Protects Ventricular Myocytes of Neonatal Rat in an In Vitro Coverslip Ischemia/Reperfusion Model
Korean Circulation Journal
;
: 234-241, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-19603
ABSTRACT
BACKGROUND AND OBJECTIVES:
The benefit of high glucose-insulin-potassium (GIK) solution in clinical applications is controversial. We established a neonatal rat ventricular myocyte (NRVM) in vitro coverslip ischemia/reperfusion (I/R) model and investigated the effects of GIK solution on suppressing reactive oxygen species (ROS) and upregulating O-GlcNacylation, which protects cells from ischemic injury. MATERIALS ANDMETHODS:
NRVMs were isolated from postnatal day 3-4 Sprague-Dawley rat pups and grown in Dulbecco's modified Eagle's medium containing high glucose (4.5 g/L), fetal bovine serum, and penicillin/streptomycin. The effects of the GIK solution on ROS production, apoptosis, and expression of O-GlcNAc and O-GlcNAc transferase (OGT) were investigated in the coverslip I/R model.RESULTS:
Covering the 24-well culture plates for 3 hr with 12 mm diameter coverslips resulted in the appropriate ischemic shock. Glucose and insulin synergistically reduced ROS production, protected NRVM dose-dependently from apoptosis, and altered O-GlcNAc and OGT expression.CONCLUSION:
The high GIK solution protected NRVM from I/R injury in vitro by reducing ROS and altering O-GlcNacylation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Choque
/
Transferasas
/
Especies Reactivas de Oxígeno
/
Ratas Sprague-Dawley
/
Apoptosis
/
Células Musculares
/
Miocitos Cardíacos
/
Glucosa
/
Insulina
Límite:
Animales
Idioma:
Inglés
Revista:
Korean Circulation Journal
Año:
2015
Tipo del documento:
Artículo
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