Your browser doesn't support javascript.
loading
HPLC analysis of Phenolic Substances and Anti-Alzheimer's Activity of Korean Quercus Species
Natural Product Sciences ; : 299-306, 2016.
Artículo en Inglés | WPRIM | ID: wpr-19612
ABSTRACT
This study aimed to establish the quantitative method to analyze the content of peroxynitrite-scavengers belonging to polyphenols in six Korean Quercus species (Quercus mongolica, Q. dentata, Q. acutissima, Q. alienta, Q. serrata, and Q. variabilis) by HPLC. The twelve peroxynitrite-scavengers, flavanols (catechins (+)-catechin, (−)-epicatechin, and (−)-epigallocatechin), flavonols (kaempferol and quercetin), flavonol glycosides (astragalin, quercitrin, and isoquercitrin), flavonol acylated glycosides (astragalin 6″-gallate and isoquercitrin 6″-gallate), gallic acid and its dimer (ellagic acid) were analyzed by HPLC. Further, anti-Alzheimer's activity was assayed in a passive avoidance testusing mice by measuring the retention latency (sec), the concentration of acetylcholine (ACh), and acetylcholinesterase (AChE) activity. Simultaneous analysis of the extracts of the six Quercus leaves was achieved on a Capcell C18 column (5 µm, 250 mm × 4.6 mm i.d.) with a gradient elution of 0.05% HAc and 0.05% HAc in CH₃CN. In the extract of Q. mongolica leaves, the content of gallic acid (32.53 mg/g), (+)-catechin (28.78 mg/g), (−)-epicatehin (22.03 mg/g), astragalin 6″-gallate (20.94 mg/g), and isoquercitrin 6″-gallate (44.11 mg/g) and peroxynitrite-scavenging activity (IC₅₀, 0.831 µg/ml) were high. This extract delayed the retention latency and inhibited acetylcholinesterase activity in scopolamine-induced memory impairment of mice, suggesting that it has anti-Alzheimer's activity.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Acetilcolinesterasa / Acetilcolina / Catequina / Cromatografía Líquida de Alta Presión / Fenol / Fagaceae / Quercus / Flavonoles / Polifenoles / Ácido Gálico Límite: Animales Idioma: Inglés Revista: Natural Product Sciences Año: 2016 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Acetilcolinesterasa / Acetilcolina / Catequina / Cromatografía Líquida de Alta Presión / Fenol / Fagaceae / Quercus / Flavonoles / Polifenoles / Ácido Gálico Límite: Animales Idioma: Inglés Revista: Natural Product Sciences Año: 2016 Tipo del documento: Artículo