Cellular uptake of magnetic nanoparticle is mediated through energydependent endocytosis in A549 cells
Journal of Veterinary Science
;
: 321-326, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-197261
ABSTRACT
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which the lung cancer cells uptake the magnetic nanoparticles. Lung cells were chosen for this study because inhalation is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken together, our results demonstrate that magnetic nanoparticles can be internalized into the cells through an energy-dependent endosomal-lysosomal mechanism.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Endosomas
/
Sacarosa
/
Temperatura
/
Materiales Biocompatibles
/
Sistemas de Liberación de Medicamentos
/
Microscopía Confocal
/
Macrólidos
/
Azida Sódica
/
Línea Celular Tumoral
/
Microscopía Electrónica de Transmisión
Límite:
Humanos
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2006
Tipo del documento:
Artículo
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