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Inhibitory Effect of Vitamin U (S-Methylmethionine Sulfonium Chloride) on Differentiation in 3T3-L1 Pre-adipocyte Cell Lines
Annals of Dermatology ; : 39-44, 2012.
Article en En | WPRIM | ID: wpr-197938
Biblioteca responsable: WPRO
ABSTRACT
BACKGROUND: S-methylmethionine sulfonium chloride was originally called vitamin U because of its inhibition of ulceration in the digestive system. Vitamin U is ubiquitously expressed in the tissues of flowering plants, and while there have been reports on its hypolipidemic effect, its precise function remains unknown. OBJECTIVE: This study was designed to evaluate the anti-obesity effect of vitamin U in 3T3-L1 pre-adipocyte cell lines. METHODS: We cultured the pre-adipocyte cell line 3T3L1 to overconfluency and then added fat differentiation-inducing media (dexamethasone, IBMX [isobutylmethylxanthine], insulin, indomethacin) and different concentrations (10, 50, 70, 90, 100 mM) of vitamin U. Then, we evaluated changes in the levels of triglycerides (TGs), glycerol-3-phosphate dehydrogenase (G3PDH), AMP-activated protein kinase (AMPK), adipocyte-specific markers (peroxisome proliferator-activated receptor gamma [PPAR-gamma], CCAAT/enhancer-binding protein alpha [C/EBP-alpha], adipocyte differentiation and determination factor 1 [ADD-1], adipsin, fatty acid synthase, lipoprotein lipase) and apoptosis-related signals (Bcl-2, Bax). RESULTS: There was a gradual decrease in the level of TGs, C/EBP-alpha, PPAR-gamma, adipsin, ADD-1 and GPDH activity with increasing concentrations of vitamin U. In contrast, we observed a significant increase in AMPK activity with increasing levels of vitamin U. The decrease in bcl-2 and increase in Bax observed with increasing concentrations of vitamin U in the media were not statistically significant. CONCLUSION: This study suggests that vitamin U inhibits adipocyte differentiation via down-regulation of adipogenic factors and up-regulation of AMPK activity.
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Texto completo: 1 Índice: WPRIM Asunto principal: Triglicéridos / Úlcera / Vitamina U / Vitaminas / Factor D del Complemento / Regulación hacia Abajo / Regulación hacia Arriba / Línea Celular / Adipocitos / Flores Idioma: En Revista: Annals of Dermatology Año: 2012 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Triglicéridos / Úlcera / Vitamina U / Vitaminas / Factor D del Complemento / Regulación hacia Abajo / Regulación hacia Arriba / Línea Celular / Adipocitos / Flores Idioma: En Revista: Annals of Dermatology Año: 2012 Tipo del documento: Article