Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs
The Korean Journal of Parasitology
;
: 59-64, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-19910
ABSTRACT
A Cryptosporidium parvum sporozoite and oocyst lambda gt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes
/
Bovinos
/
Anticuerpos Antiprotozoarios
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Proteínas Protozoarias
/
Dedos de Zinc
/
Secuencia de Aminoácidos
/
Cryptosporidium parvum
/
Antígenos de Protozoos
Límite:
Animales
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2002
Tipo del documento:
Artículo
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