Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana
Biomolecules & Therapeutics
; : 334-340, 2014.
Article
en En
| WPRIM
| ID: wpr-199227
Biblioteca responsable:
WPRO
ABSTRACT
In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-kappaB inhibitory activity of the isolated compounds was measured in TNFalpha-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited TNFalpha-stimulated NF-kappaB activation in a dose-dependent manner, with IC50 values ranging from 0.75-8.30 microM. Compounds 19 and 20 also inhibited the TNFalpha-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7-11 and 19-23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with EC50 values ranging from 0.9-10.8 microM. These results suggest the presence of potent anti-inflammatory components in P. koreana, and will facilitate the development of novel anti-inflammatory agents.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Saponinas
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ARN Mensajero
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FN-kappa B
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Factor de Necrosis Tumoral alfa
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Molécula 1 de Adhesión Intercelular
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Concentración 50 Inhibidora
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Pulsatilla
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Receptores Activados del Proliferador del Peroxisoma
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Metanol
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Células Hep G2
Idioma:
En
Revista:
Biomolecules & Therapeutics
Año:
2014
Tipo del documento:
Article