Development of Competitive ELISA for Detection of Avian Metapneumovirus Antibodies in Chicken
Journal of Bacteriology and Virology
;
: 131-143, 2010.
Artículo
en Coreano
| WPRIM
| ID: wpr-200688
ABSTRACT
Avian metapneumovirus (aMPV) causes an acute and highly contagious upper respiratory tract infection in turkeys and chickens. In this study, a competitive ELISA (C-ELISA) was developed for the detection of antibodies to aMPV in chicken sera and/or their egg yolks. This assay is based on the competitive binding of monoclonal antibody with serum antibodies to recombinant aMPV N protein expressed by a recombinant baculovirus. The C-ELISA showed specificity and sensitivity of 100% and 98.0%, respectively, when compared to the virus neutralization test. In specific pathogen-free chickens experimentally infected with aMPV SC1509 strain, the C-ELISA started to detect antibodies to aMPV as early as 5 days post infection from birds infected with aMPV, while a commercial ELISA kit detected first 10 days post infection. The C-ELISA was similar or superior to a commercial ELISA kit when serum and egg yolk samples collected from chickens on six outbreak farms were tested for diagnosis. The C-ELISA developed in the present work provides a short turnaround time and can be a useful diagnostic and screening tool for aMPV infection in the field.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Infecciones del Sistema Respiratorio
/
Esguinces y Distensiones
/
Pavos
/
Virus
/
Unión Competitiva
/
Aves
/
Ensayo de Inmunoadsorción Enzimática
/
Pruebas de Neutralización
/
Pollos
/
Tamizaje Masivo
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Año:
2010
Tipo del documento:
Artículo
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