Identification of Helicobacter pylori Strain 51 Major Outer Membrane Proteins by Quadrupole Time of Flight Mass Spectrometry
Journal of Bacteriology and Virology
;
: 103-109, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-200691
ABSTRACT
As part of an initial inquiry into the function of the outer membrane proteins (OMPs) of Helicobacter pylori Korean strain 51, we have conducted an extensive proteome analysis via quadrupole time of flight (Q-TOF) mass spectrometry (MS). Fifty one OMPs of H. pylori were purified using sarcosine and resolved via two-dimensional electrophoresis with immobilized pH gradient strips. The most abundant proteins were observed in the alkaline pI regions (6.0~11.0) at molecular masses between 10~100 KDa. Here, 15 spots were identified, representing 9 types of genes (KHP0852, KHP0853, KHP1353, KHP1017, KHP0172, KHP0076, KHP0617, KHP1069, KHP0614) from the sarcosin-insoluble fraction of H. pylori 51. These may be employed in the characterization of the OMPs of H. pylori 51, which will help to identify new potential target proteins for vaccine development and drug therapy.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sarcosina
/
Espectrometría de Masas
/
Esguinces y Distensiones
/
Proteínas
/
Helicobacter pylori
/
Helicobacter
/
Fuerza Protón-Motriz
/
Proteoma
/
Electroforesis
/
Proteínas de la Membrana
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Journal of Bacteriology and Virology
Año:
2010
Tipo del documento:
Artículo
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