LIN28B confers radio-resistance through the posttranscriptional control of KRAS
Experimental & Molecular Medicine
;
: 912-918, 2009.
Artículo
en Inglés
| WPRIM
| ID: wpr-202556
ABSTRACT
To screen the differentially expressed microRNAs related to radio-resistance, we compared the microRNA profiles of lung cancer cells with different responses to ionizing radiation (IR). Of 328 microRNAs in microarray, 27 microRNAs were differentially expressed in NCI-H460 (H460) and NCI-H1299 (H1299) cells. Among them, let-7g was down-regulated in radio-resistant H1299 cells, and the level of let-7g was higher in radio-sensitive cells like Caski, H460, and ME180 in qRT-PCR analysis than in radio-resistant cells like A549, H1299, DLD1, and HeLa. Over-expression of let-7g in H1299 cells could suppress the translation of KRAS, and increase the sensitivity to IR. When we knockdown the expression of LIN28B, an upstream regulator of let-7g, the level of mature let-7g was increased in H1299 cells and the sensitivity to IR was also enhanced in LIN28B knockdown cells. From these data, we suggest that LIN28B plays an important role in radiation responses of lung cancer cells through inhibiting let-7g processing and increasing translation of KRAS.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tolerancia a Radiación
/
Regulación Neoplásica de la Expresión Génica
/
Proteínas Proto-Oncogénicas
/
Proteínas ras
/
Perfilación de la Expresión Génica
/
MicroARNs
/
Línea Celular Tumoral
/
Proteínas de Unión al ADN
/
Neoplasias Pulmonares
Límite:
Humanos
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2009
Tipo del documento:
Artículo
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