Activation of PPARgamma induces profound multilocularization of adipocytes in adult mouse white adipose tissues
Experimental & Molecular Medicine
; : 880-895, 2009.
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| ID: wpr-202558
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WPRO
ABSTRACT
We sought to determine the effects of activation of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) on multilocularization of adipocytes in adult white adipose tissue (WAT). Male C57BL/6 normal, db/db, and ob/ob mice were treated with agonists of PPAR-gamma, PPAR-alpha, or beta3-adrenoceptor for 3 weeks. To distinguish multilocular adipocytes from unilocular adipocytes, whole-mounted adipose tissues were co-immunostained for perilipin and collagen IV. PPAR-gamma activation with rosiglitazone or pioglitazone induced a profound change of unilocular adipocytes into smaller, multilocular adipocytes in adult WAT in a time-dependent, dose-dependent, and reversible manner. PPAR-alpha activation with fenofibrate did not affect the number of locules or remodeling. db/db and ob/ob obese mice exhibited less multilocularization in response to PPAR-gamma activation compared to normal mice. Nevertheless, all adipocytes activated by PPAR-gamma contained a single nucleus regardless of locule number. Multilocular adipocytes induced by PPAR-gamma activation contained substantially increased mitochondrial content and enhanced expression of uncoupling protein-1, PPAR-gamma coactivator-1-alpha , and perilipin. Taken together, PPAR-gamma activation induces profound multilocularization and enhanced mitochondrial biogenesis in the adipocytes of adult WAT. These changes may affect the overall function of WAT.
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WPRIM
Asunto principal:
Fosfoproteínas
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Transactivadores
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Adipocitos
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Receptores Adrenérgicos beta 3
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Proteínas Mitocondriales
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Tiazolidinedionas
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PPAR alfa
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PPAR gamma
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División del Núcleo Celular
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Tejido Adiposo Blanco
Límite:
Animals
Idioma:
En
Revista:
Experimental & Molecular Medicine
Año:
2009
Tipo del documento:
Article