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The presence of CD8+ invariant NKT cells in mice
Experimental & Molecular Medicine ; : 866-872, 2009.
Artículo en Inglés | WPRIM | ID: wpr-202560
ABSTRACT
Invariant natural killer T (iNKT) cells develop in the thymus upon recognition of CD1d expressed on developing thymocytes. Although CD4 and CD8 coreceptors are not directly involved in the interaction between CD1d and the T cell receptors (TCRs) of iNKT cells, a conspicuous lack of CD8+ iNKT cells in mice raised the question of whether CD8+ iNKT cells are excluded due to negative selection during their thymic development, or if there is no lineage commitment for the development of murine CD8+ iNKT cells. To address this question, we analyzed iNKT cell-specific TCR Valpha14+ transgenic mice, where the Valpha14 transgene forces the generation of iNKT cells. This allows detailed study of the iNKT cell repertoire. We were able to identify CD8+ iNKT cells which respond to the NKT cell-specific glycolipid ligand alpha-galactosylceramide. Unlike conventional iNKT cells, CD8+ iNKT cells produce predominantly IFN-gamma but not IL-4 upon antigen stimulation. We also confirmed the presence of CD8+ iNKT cells in wild type mice. Our results suggest that CD8+ NKT cells do exist in mice, although their population size is quite small. Their Th1-skewed phenotype might explain why the population size of this subtype needs to be controlled tightly.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ratones Transgénicos / Interleucina-4 / Interferón gamma / Receptores de Antígenos de Linfocitos T alfa-beta / Linfocitos T CD8-positivos / Transgenes / Células T Asesinas Naturales / Galactosilceramidas / Ratones Endogámicos C57BL Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ratones Transgénicos / Interleucina-4 / Interferón gamma / Receptores de Antígenos de Linfocitos T alfa-beta / Linfocitos T CD8-positivos / Transgenes / Células T Asesinas Naturales / Galactosilceramidas / Ratones Endogámicos C57BL Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 2009 Tipo del documento: Artículo