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Correlation between the overexpression of epidermal growth factor receptor and mesenchymal makers in endometrial carcinoma / 부인종양
Journal of Gynecologic Oncology ; : 36-42, 2014.
Artículo en Inglés | WPRIM | ID: wpr-202950
ABSTRACT

OBJECTIVE:

The objective of this study was to evaluate the effect of overexpression of epidermal growth factor receptor (EGFR) on the expression of epithelial cell markers (E-cadherin and alpha-catenin) and mesenchymal cell markers (N-cadherin and vimentin) in endometrial carcinoma.

METHODS:

The expression of all 4 markers was evaluated in EGFR overexpressing Ishikawa cells, control Ishikawa cells, and KLE cells using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. The expression of these 4 markers was also determined in cancerous tissues of patients with endometrial carcinoma using immunohistochemical staining.

RESULTS:

Ishikawa cells transfected with EGFR showed decreased expression of E-cadherin and alpha-catenin and increased expression of N-cadherin and vimentin compared with control Ishikawa cells (p<0.01 for all). The expression of N-cadherin and vimentin was higher and the expression of E-cadherin and alpha-catenin was lower in stage II-III than stage I and in grade II-III than grade I endometrial carcinoma tissue (p<0.01 for all).

CONCLUSION:

Decreased expression of epithelial markers (E-cadherin and alpha-catenin) and increased expression of mesenchymal markers (N-cadherin and vimentin) were observed in human endometrial carcinoma tissue. These findings correlate with high EGFR expression in cultured endometrial carcinoma cells.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Vimentina / Cadherinas / Western Blotting / Reacción en Cadena de la Polimerasa / Neoplasias Endometriales / Transcripción Reversa / Factor de Crecimiento Epidérmico / Células Epiteliales / Alfa Catenina / Transición Epitelial-Mesenquimal Límite: Femenino / Humanos Idioma: Inglés Revista: Journal of Gynecologic Oncology Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Vimentina / Cadherinas / Western Blotting / Reacción en Cadena de la Polimerasa / Neoplasias Endometriales / Transcripción Reversa / Factor de Crecimiento Epidérmico / Células Epiteliales / Alfa Catenina / Transición Epitelial-Mesenquimal Límite: Femenino / Humanos Idioma: Inglés Revista: Journal of Gynecologic Oncology Año: 2014 Tipo del documento: Artículo