Expression of Cell Surface Receptors on Human Glioblastoma Xenograft Model in NOD/SCID Mouse / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
; : 52-57, 2002.
Article
en Ko
| WPRIM
| ID: wpr-203236
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: To obtain basic data for development of a glioblastoma-specific immunotoxin, the expression of variable cell surface receptors on a human glioblastoma xenograft model was evaluated, using NOD/SCID mice. MATERIALS AND METHODS: We developed a xenograft model in NOD/SCID mice implanted with a human glioblastoma cell line (U-87MG). Immunohistochemical studies were performed on implanted tumor nodules (n=8) using antibodies against CD71, EGFR, IGF-IRalpha, CXCR4 and IL-4Ralpha. RESULTS: Expression of IL-4Ralpha, in implanted tumornodules, was the highest of the cell surface receptors evaluated in this study. However, the endothelial cells in, and around, the tumor nodules also revealed immunopositivity against IL-4Ralpha. The immunoreactivity of IL-4Ralpha, and other surface receptors such as CD71, IGF-IRalpha and EGFR, was prominent in tumor nodules associated with tumor necrosis. CONCLUSION: IL-4Ralpha would be a possible target for the development of glioblastoma-specific immunotoxin, although there are limitations due to its endothelial expression.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Línea Celular
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Inmunotoxinas
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Ratones SCID
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Glioblastoma
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Receptores de Superficie Celular
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Células Endoteliales
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Xenoinjertos
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Anticuerpos
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Necrosis
Límite:
Animals
/
Humans
Idioma:
Ko
Revista:
Cancer Research and Treatment
Año:
2002
Tipo del documento:
Article