Proteomic analysis of the cellular proteins induced by adaptive concentrations of hydrogen peroxide in human U937 cells
Experimental & Molecular Medicine
;
: 374-378, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-203698
ABSTRACT
When cells are first exposed to low levels of oxidative stress, they develop a resistance to a subsequent challenge of the same stress, even at higher levels. Although some protein(s) induced by oxidative stress likely mediated this adaptive response, the nature of these proteins is unknown. In this study, the total proteins extracted from human U937 leukemia cells exposed to 50 mM H2O2 for 24 h to induce an optimal protective response were analyzed by two-dimensional polyacrylamide gel electrophoresis. H2O2 treatment induced elevation of level of 34 protein spots. An analysis of these spots by a matrix associated laser desorption/ionization time-of-flight mass spectrometry identified 28 of the H2O2-induced proteins. These include proteins involved in energy metabolism, translation and RNA processing, chaperoning or mediating protein folding, cellular signaling, and redox regulation, as well as a mitochondrial channel component, and an actin-bundling protein. Therefore, it appears that the cellular adaptation to oxidative stress is a complex process, and is accompanied by a modulation of diverse cellular functions.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Adaptación Fisiológica
/
Proteínas
/
Células Cultivadas
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Células U937
/
Proteómica
/
Peróxido de Hidrógeno
Límite:
Humanos
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2002
Tipo del documento:
Artículo
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