Transforming growth factor-beta promoted vascular endothelial growth factor release by human lung fibroblasts / 소아과
Korean Journal of Pediatrics
;
: 879-885, 2008.
Artículo
en Coreano
| WPRIM
| ID: wpr-204311
ABSTRACT
PURPOSE:
The human lung fibroblast may act as an immunomodulatory cell by providing pro-inflammatory cytokines and chemokines, which are important in airway remodeling. Vascular endothelial growth factor (VEGF) induces mucosal edema and angiogenesis. Thymus and activation regulated chemokine (TARC) induces selective migration of T helper 2 cells. We investigated whether human lung fibroblasts produced VEGF and TARC, and the effects were augmented with the co-culture of fibroblasts and human bronchial smooth muscle cells (HBSMC), and whether dexamethasone can inhibit the proliferation and the release of VEGF in lung fibroblasts.METHODS:
Human lung fibroblasts were cultured with and without HBSMC, growth-arrested in serum-deprived medium, and pretreated with dexamethasone for 16 hours. After 24-hour stimulation with platelet derived growth factor-BB (PDGF-BB) and/or transforming growth factor-beta (TGF-beta), culture supernatant was harvested for assays of VEGF and TARC. Cell proliferation was assayed using BrdU cell proliferation ELISA kit.RESULTS:
1) The release of VEGF was significantly increased after stimulation with TGF-beta, and its release was augmented when co-stimulated with PDGF and TGF-beta. 2) VEGF release induced by PDGF or TGF-beta was inhibited by dexamethasone. 3) There was no synergistic effect on the release of VEGF when human lung fibroblasts were co-cultured with HBSMC. 4) Dexamethasone did not suppress human lung fibroblasts proliferations. 5) Neither TGF-beta nor PDGF induced TARC release from lung fibroblasts.CONCLUSION:
Human lung fibroblasts may modulate airway remodeling by release of VEGF, but they have no synergistic effects when co-cultured with HBSMC. Dexamethasone suppresses VEGF release, not proliferation of lung fibroblast.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plaquetas
/
Bromodesoxiuridina
/
Dexametasona
/
Ensayo de Inmunoadsorción Enzimática
/
Citocinas
/
Factor de Crecimiento Transformador beta
/
Técnicas de Cocultivo
/
Quimiocinas
/
Miocitos del Músculo Liso
/
Factor A de Crecimiento Endotelial Vascular
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Pediatrics
Año:
2008
Tipo del documento:
Artículo
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