Regulation of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinase-1 Expression by Hypoxia in Rheumatoid and Osteoarthritis Fibroblast-Like Synoviocytes / 대한류마티스학회지
The Journal of the Korean Rheumatism Association
;
: 141-150, 2003.
Artículo
en Coreano
| WPRIM
| ID: wpr-204540
ABSTRACT
OBJECTIVE:
The rheumatoid synovium is a hypoxic environment and hypoxia has been implicated to have a role in the pathogenesis of rheumatoid arthritis (RA). In this work, we tried to investigate the effect of hypoxia on the expression of matrix metalloproteinase (MMP)-1, -3 and tissue inhibitor of metalloproteinase (TIMP)-1 in rheumatoid and osteoarthritis (OA) fibroblast-like synoviocytes (FLS).METHODS:
FLS obtained from RA and OA patients were cultured under normoxic or hypoxic condition for 48 hours. Western blot analyses for MMP-1, -3 and TIMP-1 were performed. TIMP-1 mRNA levels were measured by Northern blot analysis.RESULTS:
Hypoxia increased MMP-1 expression in rheumatoid FLS compared with normoxia. TIMP-1 expression was decreased by hypoxia in rheumatoid FLS in both protein and mRNA levels. On the other hand, hypoxia did not significantly affect MMP-1, -3 and TIMP-1 expressions in OA FLS.CONCLUSION:
These results suggest that microenvironment such as hypoxia may directly contribute to joint destruction in RA by increasing the ratio of MMP-1 to TIMP-1 production in FLS.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoartritis
/
Artritis Reumatoide
/
Membrana Sinovial
/
ARN Mensajero
/
Western Blotting
/
Northern Blotting
/
Inhibidor Tisular de Metaloproteinasa-1
/
Metaloproteinasas de la Matriz
/
Mano
/
Articulaciones
Límite:
Humanos
Idioma:
Coreano
Revista:
The Journal of the Korean Rheumatism Association
Año:
2003
Tipo del documento:
Artículo
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