Simple Purification of Adeno-Associated Virus-DJ for Liver-Specific Gene Expression
Yonsei Medical Journal
;
: 790-794, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-205734
ABSTRACT
Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediate safe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expression of a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simple large-scale preparation method that can generate 3×10(13) viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Expresión Génica
/
Cápside
/
Genoma Viral
/
Dependovirus
/
Proteínas de la Cápside
/
Células Hep G2
/
Vectores Genéticos
/
Hígado
/
Ratones Endogámicos C57BL
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2016
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS