Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis
The Korean Journal of Parasitology
;
: 121-123, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-206118
ABSTRACT
This study was designed to detect and evaluate an antigenicity of low molecular weight proteins of Fasciola hepatica in fascioliasis. Low molecular weight protein of F. hepatica was purified by ammonium sulfate precipitation and Sephacryl S-100 HR gel filtration. The protein obtained was estimated to be 8 kDa on 7.5-15% gradient sodium dodecyl sulfate gel electrophoresis. Immunoblotting studies showed that the 8 kDa protein reacted with human fascioliasis sera, but not other trematodiasis sera. This result suggests that the 8 kDa protein of F. hepatica is one of diagnostic antigens in human fascioliasis without cross-reaction with other human trematodiasis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Immunoblotting
/
Proteínas del Helminto
/
Electroforesis en Gel de Poliacrilamida
/
Fasciola hepatica
/
Fascioliasis
/
Antígenos Helmínticos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2003
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS