Immunization of BALB/c mice with Brucella abortus 2308DeltawbkA confers protection against wild-type infection
Journal of Veterinary Science
;
: 467-473, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-207358
ABSTRACT
Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308DeltawbkA) and evaluated its virulence. The survival of 2308DeltawbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308DeltawbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308DeltawbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteína Estafilocócica A
/
Virulencia
/
Brucella
/
Brucella abortus
/
Brucelosis
/
Inmunoglobulina G
/
Ovinos
/
Zoonosis
/
Interferones
/
Inmunización
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2015
Tipo del documento:
Artículo
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