Cell Death of Corneal Fibroblasts Induced by Tumor Necrosis Factor-alphaand Interferon-gamma
Journal of the Korean Ophthalmological Society
;
: 852-856, 2001.
Artículo
en Coreano
| WPRIM
| ID: wpr-207640
ABSTRACT
PURPOSE:
To evaluate the effect in the cell death of corneal fibroblasts when TNF-alphaand INF-gamma were given together.METHODS:
Fibroblasts harvested from the human cornea were cultured in DMEM, then, nothing(control Group 1), TNF-alphaonly(50 ng/ml Group 2), INF-gammaonly(1.0 x 10(3)u/ml Group 3), and a combination of both(Group 4) were added. We assessed the cell viability of the each group by the trypan blue exclusion assay at 4, 8, 12, 24, 48 hours after addition of cytokines.RESULTS:
The cell viability at 48 hour after treatment was 94.27% in group 1, 90.68%(p=0.09) in group 2, 93.31%(p=0.45) in group3, and there was no statistical difference among the groups. Statistically signi-ficant decrease of the cell viability was achieved in group 4(82.86%, p=0.002).CONCLUSIONS:
Cell death of human corneal fibroblasts had been increased after treatment with a combination of TNF-alphaand INF-gamma. These findings suggest that there could be some kind of interaction among the cytokines.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Azul de Tripano
/
Supervivencia Celular
/
Citocinas
/
Interferón gamma
/
Factor de Necrosis Tumoral alfa
/
Muerte Celular
/
Córnea
/
Fibroblastos
/
Necrosis
Límite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Año:
2001
Tipo del documento:
Artículo
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