Effect of Aminoglycosides on Leukochemotaxis in Rats

ABSTRACT

In order to study the effects of aminoglycosides on leukochemotaxis in rats, the degree of leukocyte infiltration and differential leukocyte count were observed at the experimentally inflamed tissue by incision of the skin and the application of endotoxin subcutaneously after the administration of gentamicin and streptomycin respectively. The results obtained were as follows. 1) In the control group treated with physiological, saline, the infiltration of leukocytes was relatively scant in the first nne hour, conspicuously increased three hours later, and persisted for 24 hours. 2) In the gentamicin and streptomycin treated groups, infiltration of leukocytes was srnall in number in the first hour. There after it gradually increased over a period of time. However it was a little less in the gentamicin and streptomycin injected group compared with the control. 3)In the control group, about 90% of the infiltrated cells was neutrophils up to three hours. However 24 hours later, the neutrophils reached about 40%. On the other hand, monocytes and lymphocytes were 4% in the first hour, and after 24 hours the monocytes was 19 to 24% and lymphocytes were 31 to 36%. 4) In the gentamicin and streptomycin injected grougs, about 90% of the infiltrated cells was neutrophils for the first three hours. The numbers gradually decreased to 56-66% and 51~54% after 24 hours, respectively. The percentage of both monocytes and lymphocytes was 2~4% after ome hour, and increased to 15~29 and 17~29% after 24 houre, respectively.