Proliferation of Corneal Endothelial Cells by Delivery of SV40 Large T Antigen
Journal of the Korean Ophthalmological Society
;
: 1331-1335, 2001.
Artículo
en Coreano
| WPRIM
| ID: wpr-209889
ABSTRACT
PURPOSE:
To determine whether the delivery of the SV40 large T-antigen is a feasible method for transiently inducing proliferation of corneal endothelial cells, we delivered liposome-protein complex into bovine corneal endothelial cells(BCEC).METHOD:
SV40 large T-antigen protein was introduced into BCEC and positive cells were identified by immunohistochemistry. Quiescent BCECs were double-labeled using BrdU as a measure of de novo DNA synthesis and the Ki-67 was detected by standard immunohistochemical methods.RESULT:
The treatment of quiescent BCECs with large T antigen caused an increase in BrdU incorporation and Ki-67 expression. It was tested by time-course study.CONCLUSION:
This finding suggests that liposome-mediated delivery of transforming proteins could be a method to transiently induce corneal endothelial cell proliferation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Bromodesoxiuridina
/
ADN
/
Inmunohistoquímica
/
Células Endoteliales
/
Proliferación Celular
/
Antígenos Virales de Tumores
Tipo de estudio:
Estudio pronóstico
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Año:
2001
Tipo del documento:
Artículo
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