Transplantation of a Scaffold-Free Cartilage Tissue Analogue for the Treatment of Physeal Cartilage Injury of the Proximal Tibia in Rabbits
Yonsei Medical Journal
;
: 441-448, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-21011
ABSTRACT
PURPOSE:
The purpose of this study was to investigate the effects of transplantation of an in vitro-generated, scaffold-free, tissue-engineered cartilage tissue analogue (CTA) using a suspension chondrocyte culture in a rabbit growth-arrest model. MATERIALS ANDMETHODS:
We harvested cartilage cells from the articular cartilage of the joints of white rabbits and made a CTA using a suspension culture of 2x107 cells/mL. An animal growth plate defect model was made on the medial side of the proximal tibial growth plate of both tibias of 6-week-old New Zealand white rabbits (n=10). The allogenic CTA was then transplanted onto the right proximal tibial defect. As a control, no implantation was performed on the left-side defect. Plain radiographs and the medial proximal tibial angle were obtained at 1-week intervals for evaluation of bone bridge formation and the degree of angular deformity until postoperative week 6. We performed a histological evaluation using hematoxylin-eosin and Alcian blue staining at postoperative weeks 4 and 6.RESULTS:
Radiologic study revealed a median medial proximal tibial angle of 59.0degrees in the control group and 80.0degrees in the CTA group at 6 weeks. In the control group, statistically significant angular deformities were seen 3 weeks after transplantation (p<0.05). On histological examination, the transplanted CTA was maintained in the CTA group at 4 and 6 weeks postoperative. Bone bridge formation was observed in the control group.CONCLUSION:
In this study, CTA transplantation minimized deformity in the rabbit growth plate injury model, probably via the attenuation of bone bridge formation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tibia
/
Trasplante Autólogo
/
Trasplante Homólogo
/
Cartílago
/
Células Cultivadas
/
Trasplante Óseo
/
Técnicas de Cultivo de Célula
/
Condrocitos
/
Ingeniería de Tejidos
/
Trasplante de Células Madre Mesenquimatosas
Límite:
Animales
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2016
Tipo del documento:
Artículo
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