Different Expressions of HIF-1alpha, Bcl-2 and Baxin DU145 Prostate Cancer Cells Transplanted in Nude Mouse between X-Ray and Neutron Irradiation / 대한방사선종양학회지
The Journal of the Korean Society for Therapeutic Radiology and Oncology
;
: 218-227, 2009.
Artículo
en Inglés
| WPRIM
| ID: wpr-21051
ABSTRACT
PURPOSE:
To investigate the radiobiologic effects of neutron and X-ray irradiation on DU-145 prostate carcinoma cells by identifying the differences of HIF-1alpha expression and apoptosis. MATERIALS ANDMETHODS:
Nude mice were injected with the human prostate cancer cell line, DU-145, and then irradiated with 2 Gy and 10 Gy X-rays, or 0.6 Gy and 3.3 Gy neutrons, respectively. The mice were sacrificed at 24 hours and 120 hours after irradiation. The expression levels of HIF-1alpha, Bcl-2 and Bax were compared with immunohistochemical staining and western blotting. The apoptotic indexes were compared with the Terminal deoxynucleotidyl biotin-dUTP nick and labeling (TUNEL) assay.RESULTS:
At day 1, HIF-1alpha and Bcl-2 expression decreased, while Bax expression and the number of TUNEL positive cells increased in neutron irradiated groups for the control and X-ray irradiated groups. The Bcl-2/Bax ratio was significantly lower in the neutron irradiated groups regardless of dose (p=0.001). The same pattern of the differences in the expressions of the HIF-1alpha, Bcl-2, Bax, Bcl-2/Bax ratio, and apoptotic indexes were indentified at day 5. HIF-1alpha expression was related with Bcl-2 (p=0.031), Bax (p=0.037) expressions and the apoptotic indexes (p=0.016) at day 5.CONCLUSION:
Neutron irradiation showed a decrease in HIF-1alpha, Bcl-2 expression, and Bcl-2/Bax ratio, but increased Bax expression regardless of dose. This study suggests that the differences radiobiological responses between photon and neutron irradiation may be related to different HIF-1alpha expression and subsequent apoptotic protein expressions.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Próstata
/
Neoplasias de la Próstata
/
Línea Celular
/
Western Blotting
/
Apoptosis
/
Trasplantes
/
Etiquetado Corte-Fin in Situ
/
Ratones Desnudos
/
Neutrones
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
The Journal of the Korean Society for Therapeutic Radiology and Oncology
Año:
2009
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS