Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi
Journal of Korean Medical Science
;
: 866-870, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-210701
ABSTRACT
Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to the survival of the pathogen. However, there have been no reports confirming whether O. tsutsugamushi indeed produce OMVs. O. tsutsugamushi boryong was cultured in ECV-304 cells for the purification of OMVs. Western blot analysis and immunoenrichment using anti-O. tsutsugamushi monoclonal antibody and electron microscopy were employed for identification and characterization of OMVs. We confirm the presence of OMVs derived from O. tsutsugamushi, and also found that those OMVs contain a major surface antigen of 56-kDa protein and variant immunogenic antigens.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Orientia tsutsugamushi
/
Tifus por Ácaros
/
Microscopía Electrónica
/
Línea Celular
/
Membrana Celular
/
Vesículas Secretoras
/
Anticuerpos Monoclonales
/
Antígenos Bacterianos
/
Antígenos de Superficie
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Journal of Korean Medical Science
Año:
2015
Tipo del documento:
Artículo
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